Ä«Å×°í¸®
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Others
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CAT.NO
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LGP-60-001
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PRODUCT
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MHC Class I Polypeptide-Related Sequence A(MIC-A, human)
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SIZE
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50ug, 250ug, 1mg
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PRICE
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KRW 285,000, 1,000,000, 3,300,000
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Technical Parameters
Species |
9 |
Accession |
Q29983 |
GeneID |
100507436 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 32.8 kDa, a single non-glycosylated polypeptide chain containing 284 amino acids. |
Quantity |
50µg/250µg/1000µg |
AA Sequence |
EPHSLRYNLT VLSWDGSVQS GFLAEVHLDG QPFLRYDRQK CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ NLETEEWTVP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLESGVVLRR TVPPMVNVTR SEASEGNITV TCRASSFYPR NIILTWRQDG VSLSHDTQQW GDVLPDGNGT YQTWVATRIC RGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSHW |
Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The specific activity is determined by binding MICA antibody in ELISA. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 30 % Acetonitrile, 0.1% TFA. |
Endotoxin |
Less than 1 EU/µg of rHuMIC-A as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration less than 0.2 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Yao Z, Volgger A, Helmberg W, et al. 1999. Eur J Immunogenet, 26: 225-32. 2. Li P, Willie ST, Bauer S, et al. 1999. Immunity, 10: 577-84. 3. Petersdorf EW, Shuler KB, Longton GM, et al. 1999. Immunogenetics, 49: 605-12. 4. Komatsu-Wakui M, Tokunaga K, Ishikawa Y, et al. 1999. Immunogenetics, 49: 620-8. 5. Gambelunghe G, Ghaderi M, Cosentino A, et al. 2000. Diabetologia, 43: 507-14. |
Background |
MIC-A (MHC class I chain-related gene A) is a single-pass type I member protein. It is expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Additionally, MIC-A can be induced by bacterial and viral infections. It shares 85% amino acid identity with MIC-B and they are distantly related to the MHC class I proteins. Because they possess three extracellular Ig-like domains, but unlike classical MHC class I molecules. They do not form a heterodimer with beta2 microglobulin, but bind as a monomer to a KLRK1/NKG2D that is an activating receptor expressed on NK cells, NKT cells, ¥ã¥ä T cells, and CD8+ ¥á¥â T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MIC-A recognition plays an important role in tumor surveillance, viral infections, and autoimmune diseases. |
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