Lentivirus Packaging Service

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  • À̹ÌÁö
  • Lentiviral transfer vector Ŭ·Î´×ºÎÅÍ ¹ÙÀÌ·¯½º »ý»ê±îÁö (Lentiviral transfer vector ¹«»óÁ¦°ø, cDNA Ŭ·Ð ±¸ÀÔºñ ¶Ç´Â À¯ÀüÀÚ ÇÕ¼ººñ º°µµ)
  • ´Ù¾çÇÑ Cancer cell°ú Primary cell¿¡¼­ ¾ÈÁ¤ÀûÀ¸·Î cDNA, shRNA, miRNA ¹ßÇöÀ¯µµ
  • 2 & 3 Generation VSV-G pseudo-envelop type lentivirus packaging ¸ðµÎ °¡´É
  • ´Ù¾çÇÑ Promoter, Reporter gene ±×¸®°í Antibiotic resistant gene ¼±Åð¡´É
  • High-titer º¸Àå (1 x 10^8 ~ 10^9 IFU/ml)
  • ÇØ¿Ü OEM »ý»êÀÌ ¾Æ´Ñ ±¹³» ·»Æ¼¹ÙÀÌ·¯½º »ý»ê½Ã½ºÅÛ: LMO ¼öÀԽŰí ÀýÂ÷°¡ ÇÊ¿ä¾øÀ½
  • High stability lentivirus: ÀÚ»ç Á¦Ç°ÀÎ Lenti-Con¢â Reagent¿Í HIV-Safe¢â Manager¸¦ Á¦Ç° Á¦ÀÛ¿¡ È°¿ëÇÏ¿© Ÿ»çÁ¦Ç° ´ëºñ ³ôÀº ¾ÈÁ¤¼º º¸Àå

¼­ºñ½º ÁøÇà °úÁ¤

¼­ºñ½º ÁøÇà°úÁ¤ À̹ÌÁö ¼­ºñ½º ÁøÇà°úÁ¤ À̹ÌÁö

°¡°Ý ¹× ¼Ò¿ä±â°£

Service Type Titer Volume Price (¿ø) ¼Ò¿ä±â°£
Pilot scale > 1 x 10^8 IFU/ml 0.25 ml 400,000 2 ~ 3ÁÖ
Medium scale > 1 x 10^8 IFU/ml
> 1 x 10^9 IFU/ml
1.0 ml
0.1 ml
1,000,000 2 ~ 3ÁÖ
Large scale > 1 x 10^8 IFU/ml
> 1 x 10^9 IFU/ml
10 ml
1.0 ml
°ßÀû¹®ÀÇ 2 ~ 3ÁÖ
Vector cloning (< 2 kb) Lentiviral vector ¹«»óÁ¦°ø °ßÀû¹®ÀÇ + 2ÁÖ

* ¸ðµç ·»Æ¼¹ÙÀÌ·¯½º Á¦Ç°±º¿¡´Â °í°´ÀÇ Á¦Ç°»ç¿ë ÆíÀǸ¦ À§ÇØ transduction enhancerÀÎ Lenti-TD MAX°¡ ¹«»óÁ¦°ø µË´Ï´Ù.

* º¤ÅÍŬ·Î´×ºÎÅÍ ¹ÙÀÌ·¯½º Á¦ÀÛ±îÁö all-in-one ¼­ºñ½º ÀÌ¿ë ½Ã ÃÖÁ¾ ¹ÙÀÌ·¯½º Á¦Ç° ¿Ü¿¡ Á¦ÀÛµÈ Lenti-viral transfer vector¿Í Àüü¼­¿­ÀÌ Æ÷ÇÔµÈ sequence map ÆÄÀÏÀÌ µ¿½Ã Á¦°øµË´Ï´Ù.

Lentivector Cloning ¼±ÅÃ¿É¼Ç (°ßÀû¿äû ½Ã ÇØ´ç»çÇ×À» ¿äûÇØ ÁÖ¼¼¿ä)


  • Two promoter system

    two promter system

    * Promoter 1 : CMV, MSCV, EF1a, PGK Áß ¼±Åð¡´É

    * Promoter 2 : EF1a, PGK Áß ¼±Åð¡´É (Promoter 1°ú 2¿¡ µ¿ÀÏÇÑ promoter ¼±ÅúҰ¡)

    * Your Gene : Genebank Accession Number or cDNA sequence

    * Reporter Gene : GFP, RFP, PuroR, BleoR, BSDR Áß ¼±Åð¡´É



  • One promter 2A system

    one promter 2A system

    * Promoter 1 : CMV, MSCV, EF1a, PGK Áß ¼±Åð¡´É

    * Your Gene 1 & 2 : Genebank Accession Number or cDNA sequence

    * Reporter Gene : GFP, RFP, PuroR, BleoR, BSDR Áß ¼±Åð¡´É



  • One promoter IRES system

    One promoter IRES system

    * Promoter 1 : CMV, MSCV, EF1a, PGK Áß ¼±Åð¡´É

    * Your Gene 1 & 2 : Genebank Accession Number or cDNA sequence

    * Reporter Gene : GFP, RFP, PuroR, BleoR, BSDR Áß ¼±Åð¡´É



  • shRNA Expression system

    shRNA Expression system

    * Promoter : U6 promoter ±âº» »ç¿ë

    * Your shRNA : À¯Àú º¸À¯ shRNA ¼­¿­À̳ª ¼­¿­À» ¸ð¸¦ °æ¿ì Target GeneÀÇ Genebank Accession Number¸¦ Á¦°øÇϸé shRNA µðÀÚÀÎ °¡´É

    * Reporter Gene : GFP, PuroR Áß ¼±Åð¡´É



  • miRNA Expression system

    miRNA Expression system

    * Promoter : CMV promoter ±âº» »ç¿ë

    * Your shRNA : miRNA À̸§À» ¾Ë·ÁÁֽøé pre-miRNA ¼­¿­ÀÌ Æ÷ÇÔµÈ 450 ~ 500 bp genomic sequence¸¦ ÇÕ¼ºÇÏ¿© Ŭ·Î´× ÇÔ.

    * Reporter Gene : GFP, PuroR Áß ¼±Åð¡´É

Promoter ¼±Åð¡À̵å

Promoter Expression level Application
CMV High In most cell lines
MSCV High Hematopoietic and stem cells
EF1a Medium Most cell types including primary cells and stem cells
PGK Medium Most cell types including primary cells and stem cells
U6 High shRNA specific expression

Á¦Ç°ÀÇ QC

  • ¹ÙÀÌ·¯½º ³óµµÃøÁ¤: RT-qPCR titration

¹ÙÀÌ·¯½º ¿ª°¡º¸Áõ ¿¹¿ÜÀÎ °æ¿ì

  • ·»Æ¼¹ÙÀÌ·¯½º º¤ÅÍ·Î µµÀÔµÈ insert ±æÀÌ°¡ 4 kb ÀÌ»óÀÎ °æ¿ì
  • Insert À¯ÀüÀÚ°¡ ¹ÙÀÌ·¯½º packaging¿¡ ¿µÇâÀ» ÁÖ´Â À¯ÀüÀÚÀÎ °æ¿ì
  • ¹ÙÀÌ·¯½º packaging ¼¼Æ÷¿¡ insert À¯ÀüÀÚ°¡ µ¶¼ºÀ» À¯¹ßÇÏ´Â °æ¿ì
  • Secondary structure À¯¹ß¼­¿­ ¹× ±× ¿Ü ¾Ë·ÁÁöÁö ¾ÊÀº ¸ÞÄ¿´ÏÁò¿¡ ÀÇÇØ ¹ÙÀÌ·¯½º packaging¿¡ ºÎÁ¤ÀûÀÎ ¿µÇâÀ» À¯¹ßÇÏ´Â ¼­¿­ÀÎ °æ¿ì
  • °í°´ Á¦°ø plasmid ³» ¹ÙÀÌ·¯½º packaging °ü·Ã ÁÖ¿äÀÎÀÚ°¡ °á¿©µÈ °æ¿ì
  • °ËÁõµÈ envelop ÀÌ¿Ü¿¡ °í°´ Á¦°ø ´Ù¸¥ pseudo-envelop type ·»Æ¼¹ÙÀÌ·¯½º »ý»êÀ» ½ÃµµÇÏ´Â °æ¿ì
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