Bacterial Expression

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  • ´Ü¹éÁú °ú¹ßÇö¿¡ ÃÖÀûÈ­µÈ Codon Optimization ¼­ºñ½º Á¦°ø
  • À¯ÀüÀÚ ¼ºÇâ¿¡ ¸Â´Â ÃÖÀûÀÇ Host-Bacterial Expression Vector Á¶ÇÕ Á¦°ø
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  • Codon optimizationÀº Ÿ°Ù ´Ü¹éÁú ¹ßÇöÀ» È¿°úÀûÀ¸·Î ³ô¿©ÁÙ ¼ö ÀÖÀ¸¸ç ´Ü¹éÁú ±â´É Çâ»óÀ» °¡´ÉÇÏ°Ô ÇÔ. ¶ÇÇÑ º» ÀÛ¾÷À» ÅëÇØ Á¶Á÷ ÀûÇÕ¼ºÀ» Áõ°¡½Ãų »Ó¸¸ ¾Æ´Ï¶ó ¹ßÇö¿¡ ÀúÇØ¿ä¼ÒÀÎ 2Â÷ ±¸Á¶ ¹× ¹Ýº¹±¸Á¶ Çü¼º °¡´É¼ºÀ» Á¦°ÅÇÒ ¼ö ÀÖÀ½.

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  • Codons Optimization

  • Gene synthesis
    Subcloning
    Transformation

  • Small scale expression & Purification test

  • Scale-up Production

  • Purification

  • QC

ÁøÇà´Ü°è ÁøÇà³»¿ë ¼Ò¿ä±â°£
Gene synthesis / Subcloning
  • À¯ÀüÀÚ codon optimization ¹× Synthesis
  • E.coli Àü¿ë ¹ßÇöº¤ÅÍ ¼±Á¤ ÈÄ subcloning
  • DNA QC
2-3 ÁÖ
E.coli transformation / Small scale protein expression test
  • ´Ü¹éÁú ¹ßÇöÀ» À§ÇÑ ÃÖÀû E.coli strain ¼±Á¤
  • Á¦Á¶µÈ plasmid¸¦ ÀÌ¿ëÇØ E.coli transformation
  • ´Ü¹éÁú ¹ßÇö È®ÀÎ ÈÄ ¹ßÇöÁ¶°Ç ÃÖÀûÈ­
1 ÁÖ
Large scale protein purification/QC & Quantitation
  • ÃÖÀûÈ­µÈ Á¶°ÇÀ¸·Î ¹è¾çÀ» scale-up
  • ´Ü¹éÁú Á¤Á¦¸¦ ÅëÇØ °í³óµµÀÇ ´Ü¹éÁú È®º¸
  • SDS-PAGE/ Western blot ºÐ¼®¹ýÀ» ÀÌ¿ëÇÑ Á¤Á¦µÈ ´Ü¹éÁúÀ» °ËÃâÇÏ°í ¼øµµ¸¦ ºÐ¼®
  • BCA ºÐ¼®À» ÅëÇÑ Á¤·®
2-4 ÁÖ

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  • Protein functional study
  • In vitro protein binding assay
  • Enzyme preparation
  • Antigen preparation for antibody production
  • Standard antigen for ELISA design
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