Ä«Å×°í¸®
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Enzymes & Inhibitors
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CAT.NO
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LGP-45-009
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PRODUCT
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Beta-lactamase TEM-1
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SIZE
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5mg, 10mg, 50mg
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PRICE
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KRW 285,000, 570,000, 2,676,000
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.
Technical Parameters
Source |
Escherichia coli. |
Molecular Weight |
Approximately 28.9 kDa, a single non-glycosylated polypeptide chain containing 264 amino acids. |
Quantity |
5mg/10mg/50mg |
AA Sequence |
MHPETLVKVK DAEDQLGARV GYIELDLNSG KILESFRPEE RFPMMSTFKV LLCGAVLSRV DAGQEQLGRR IHYSQNDLVE YSPVTEKHLT DGMTVRELCS AAITMSDNTA ANLLLTTIGG PKELTAFLHN MGDHVTRLDR WEPELNEAIP NDERDTTMPA AMATTLRKLL TGELLTLASR QQLIDWMEAD KVAGPLLRSA LPAGWFIADK SGAGERGSRG IIAALGPDGK PSRIVVIYTT GSQATMDERN RQIAEIGASL IKHW |
Purity |
> 95 % by SDS-PAGE. |
Biological Activity |
Fully biologically active when compared to standard. One unit of enzyme activity is defined as the amount of enzyme which will hydrolyze 1.0 ¥ìmol of benzyl penicillin in presence of EDTA at pH 7.0 and at 25 ¡ÆC. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 100 mM Tris, pH 7.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Reference |
1. Bush K, Jacoby GA, Medeiros AA. 1995. Antimicrob Agents Chemother. 39:1211-33. 2. Ambler RP. 1980. Philos Trans R Soc Lond B Biol Sci. 289:321-31. 3. Leinberger DM, Grimm V, Rubtsova M, et al. 2010. J Clin Microbiol. 48:460-71. |
Background |
Beta-lactamases are enzymes produced by some bacteria and are responsible for their resistance to beta-lactam antibiotics like penicillins, cephamycins, and carbapenems. The lactamase enzyme breaks the ¥â-lactam ring open and deactivates the molecule's antibacterial properties because of a common element in these antibiotics molecular structure: a four-atom ring known as a beta-lactam. TEM-1 is the most commonly-encountered beta-lactamase in gram-negative bacteria. Up to 90 % of ampicillin resistance in E. coli is due to the production of TEM-1. Also responsible for the ampicillin and penicillin resistance that is seen in H. influenzae and N. gonorrhoeae in increasing numbers. Based upon different combinations of changes, currently 140 TEM-type enzymes have been described. Recombinant beta-lactamase TEM-1 contains 264 amino acids residues. |
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