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카테고리
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Chemokines
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CAT.NO
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LGP-22-023
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PRODUCT
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MCP-5/CCL12, Mouse (Murine Monocyte Chemotactic Protein-5/CCL12)
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SIZE
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20ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
| Synonyms |
MCP-1-related Chemokine, Small-inducible Cytokine A12 |
| Accession |
Q62401 |
| GeneID |
20293 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids. |
| Quantity |
20µg/100µg/500µg |
| AA Sequence |
GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG |
| Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/μg of rMuMCP-5/CCL12 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Reference |
1. Sarafi MN, Garcia-Zepeda EA, MacLean JA, et al. 1997. J Exp Med. 185:99-109.
2. Jia GQ, Gonzalo JA, Lloyd C, et al. 1996. J Exp Med. 184:1939-51. |
| Background |
Murine CCL12 is belonging to the CC chemokine family. It shows 66 % amino acid sequence identity to human MCP-1. CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, it will also chemoattract eosinophils. CCL12 can induce a calcium flux in human HEK-293 cells transfected with murine CCR2, but can not induce a calcium flux in HEK-293 cells transfected with CCR1, CCR3, or CCR5. That shows the functional receptor of CCL12 is CCR2. |
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첨부파일
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