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카테고리
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Chemokines
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CAT.NO
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LGP-22-021
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PRODUCT
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MIP-1γ/CCL9/CCL10, Mouse (Murine Macrophage Inflammatory Protein-1 gamma/CCL9/CCL10)
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SIZE
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20ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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Technical Parameters
| Synonyms |
MIP-1-gamma, MRP-2, Small-inducible Cytokine A9 |
| Accession |
P51670 |
| GeneID |
20308 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 11.6 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids. |
| Quantity |
20µg/100µg/500µg |
| AA Sequence |
QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q |
| Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/μg of rMuMIP-1γ/CCL9/CCL10 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| SDS-PAGE |
 |
| Reference |
1. Youn BS, Jang IK, Broxmeyer HE, et al. 1995. J Immunol. 155:2661-7.
2. Berahovich RD, Miao Z, Wang Y, et al. 2005. J Immunol. 174:7341-51.
3. Lean JM, Murphy C, Fuller K, et al. 2002. J Cell Biochem. 87:386-93. |
| Background |
Murine CCL9/10 is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 shows a 51 % sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Murine CCL9/10 is 75 % amino acid identical to rat CCL9/10. |
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