|
카테고리
|
Chemokines
|
|
CAT.NO
|
LGP-22-016
|
|
PRODUCT
|
MIP-1β/CCL4, Mouse (Murine Macrophage Inflammatory Protein-1 beta/CCL4)
|
|
SIZE
|
10ug, 100ug, 500ug
|
|
PRICE
|
KRW 285,000, 880,000, 2,569,000
|
Technical Parameters
| Synonyms |
ACT-2, MIP-1-beta, Protein H400, SIS-gamma, Small-inducible cytokine A4 |
| Accession |
P14097 |
| GeneID |
20303 |
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids. |
| Quantity |
10µg/100µg/500µg |
| AA Sequence |
APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN |
| Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 20-100 ng/ml. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/µg of rMuMIP-1β/CCL4 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Reference |
1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9.
2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31.
3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94. |
| Background |
Chemokine (C-C motif) ligand 4, also known as Macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant murine CCL4 contains 69 amino acids and it shares 77 % and 86 % a.a. sequence identity with human and rat CCL4. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells. |
|
|
첨부파일
|
|
|
|
|
|
