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카테고리 Chemokines
CAT.NO LGP-22-014
PRODUCT Fractalkine/CX3CL1, Mouse (Murine Fractalkine/CX3CL1)
SIZE 20ug, 100ug, 500ug
PRICE KRW 285,000, 880,000, 2,569,000
Technical Parameters
Accession O35188
GeneID 20312
Source Escherichia coli.
Molecular Weight Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids and comprises only the chemokine domain of Murine Fractalkine.
Quantity 20µg/100µg/500µg
AA Sequence QHLGMTKCEI MCGKMTSRIP VALLIRYQLN QESCGKRAIV LETTQHRRFC ADPKEKWVQD AMKHLDHQAA ALTKNG
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human peripheral blood lymphocytes (PBL) is less than 0.5 μg/ml, corresponding to a specific activity of > 2000 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 0.1 EU/µg of rMuFractalkine/CX3CL1 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Usage This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
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