Ä«Å×°í¸®
|
Cytokines & Growth Factor
|
CAT.NO
|
LGP-14-005
|
PRODUCT
|
IL-2, Liquid, Rat (Interleukin-2, Liquid)
|
SIZE
|
20ug, 100ug, 500ug
|
PRICE
|
KRW 285,000, 807,000, 2,390,000
|
Technical Parameters
Synonyms |
T-cell Growth Factor, CGF |
Accession |
P17108 |
GeneID |
116562 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids. |
Quantity |
20µg/100µg/500µg |
AA Sequence |
APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ |
Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 ¡¿ 106 IU/mg. |
Physical Appearance |
Sterile Colorless liquid. |
Formulation |
A 0.2 ¥ìm filtered concentrated sterile solution in 10 mM sodium citrate, pH 4.0, 30 % glycerol. |
Endotoxin |
Less than 1 EU/¥ìg of rRtIL-2, Liquid as determined by LAL method. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 6 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 3 months, -20 to -70 ¡ÆC under sterile conditions after opening. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Reference |
1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79. 2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10. 3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51. 4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25. 5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6. |
Background |
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, ¥ã¥ä T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2R¥á, 75 kDa IL2R¥â, 64 kDa common gamma chain ¥ãc/IL2R¥ã) that are present on the cell surface in varying preformed complexes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 82 %, 66 %, 66 %, 60 % and 54 % amino acid sequence identity with mouse, human, rhesus macaque, pig and equus caballus IL-2, respectively. |
|
÷ºÎÆÄÀÏ
|
|
|
|
|